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Prevacuole compartments are late endosomes. Quantitation of Ste2p and CPY in prevacuole compartments after exposure to α-factor at 25 or 37°C. For each time point 300 cell sections were observed, and the number of CPY- or Ste2p-containing endosomes was counted. (A) Percentage of cell sections that were observed with CPY or with Ste2p antibody localization. (B) Average number of marker-positive (Ste2p or CPY) endosomes that were observed within those cell sections.
