Figure 6.
Comparison of GLUT1 and GLUT1CBP mRNA expression in various tissues and cells. Poly(A)+ RNA (2 μg) isolated from each of the indicated rat tissues or cell monolayers was resolved on a denaturing agarose gel and then transferred to a nylon membrane. Using 32P-labeled antisense RNA probes, we first hybridized the message with the GLUT1CBP probe and then stripped and hybridized with the GLUT1 probe. Bound probe was detected in both cases by phosphorimager analysis. In vitro transcribed GLUT1CBP (0.1 ng) is included as a standard.