Figure 1.

Induction of Mac-1-dependent monocyte adhesion to ICAM-1 by CC chemokines. (A–C) Kinetics (A), dose dependence (B), and mAb inhibition (C) of chemokine-stimulated monocyte binding to ICAM-1. (D) Effect of PMA, CBR-LFA1/2 mAb, and Mn²⁺ on monocyte binding to ICAM-1. Monocytes were subjected to adhesion assays on ICAM-1 at 37°C in the presence of MIP-1α (10 ng/ml), RANTES (100 ng/ml), and MCP-1 (100 ng/ml) (A and C), MCP-1 at indicated concentrations (B), or PMA (10 ng/ml), activating β2 mAb CBR-LFA1/2 (1 μg/ml), or Mn²⁺ (1 mM) (D) for indicated periods (A) or for 30 min (B–D). Cells were preincubated with mAbs to αL (TS1/22), αM (CBRM1/29), or isotype control wells with ICAM-1 mAb R6.5 (B and C). Data are mean ± SD of three independent experiments performed in triplicate.