Figure 2.

Early, transient up-regulation of LFA-1 activity by MCP-1 and SDF-1α. Monocytes (A) and αL- or mock-transfected Jurkat J-β2.7 cells either coexpressing CCR2 (B) or not (C) were stimulated with MCP-1 (100 ng/ml) or SDF-1α (3 μg/ml) for indicated periods, in the presence of the CD11α activation epitope reporter mAb 24 or isotype control at 37°C, and then stained with FITC-conjugated mouse IgG mAb on ice and analyzed by flow cytometry with appropriate light scatter gates. Expression of the 24 epitope is reported as mean fluoresence intensity (percentage of isotype control). Data are mean ± SD of three separate experiments.