Skip to main content
. 1999 Apr;10(4):861–873. doi: 10.1091/mbc.10.4.861

Figure 5.

Figure 5

Differential regulation of LFA-1 and Mac-1 avidity by SDF-1α is mediated by the α subunit cytoplasmic domain. (A and B) Surface expression of LFA-1 and Mac-1 extracellular regions on J-β2.7 cells transfected with chimeric α subunit cytoplasmic domain exchanges LE and ME. J-β2.7αLE and J-β2.7αME transfectants were stained with mAbs to αL (TS1/22) or αM (CBRM1/29), respectively (solid line), and isotype control (dotted line). Shown is one representative experiment. (C–F) Adhesion of J-β2.7 transfectants to ICAM-1. Cells were subjected to adhesion assays on ICAM-1 at 37°C with or without stimulation with PMA (100 ng/ml) for 30 min (C and D) or in the presence of SDF-1α (3 μg/ml) for the indicated periods (E and F). For mAb inhibition assays, cells were preincubated with saturating concentrations of mAbs for 30 min on ice. Data are mean ± SD of three independent experiments performed in triplicate. *, p < 0.05 versus unstimulated control.