Figure 8.

Role of β2 integrins in monocyte transendothelial chemotaxis. Inhibition by mAb of monocyte chemotaxis in response to MCP-1 at 100 (A) or 1 (B) ng/ml is shown. Monocytes were pretreated with mAb to β2 (TS1/18), αL (TS1/22), αM (CBRM-1/29, or nonblocking OKM-1) on ice, and endothelial monolayers were treated with mAb to ICAM-1 (R6.5), ICAM-2 (CBR-IC2/2), or isotype control for 20 min and washed. Spontaneous migration was 2.5 ± 0.6% of input, and migration in response to MCP-1 at 100 and 1 ng/ml (isotype control) was 45.4 ± 4.9 and 14.5 ± 2.4% of input, respectively. Data are mean ± SD of three independent experiments performed in duplicate.