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. 1999 Apr;10(4):875–890. doi: 10.1091/mbc.10.4.875

Figure 1.

Figure 1

A-ALP is processed rapidly in a vps35-ts strain after shifting to the nonpermissive temperature. Wild-type (SNY36–9A), vps35Δ (SNY79), and vps35-ts (PBY4) yeast strains carrying a CEN plasmid directing expression of A-ALP (pSN55) were analyzed. The strains were grown for several doublings at 22°C, preincubated at 35°C for 10 min, and then pulsed with [35S]methionine/cysteine for 10 min before unlabeled amino acids were added to initiate the chase. At the indicated times A-ALP was immunoprecipitated from the cultures and analyzed by SDS-PAGE and fluorography. The positions of unprocessed pro-A-ALP (pA-ALP) and mature A-ALP (mA-ALP) are indicated.