Figure 4.

Phenotypes resulting from a loss of Vps35p function combined with a retrieval-defective, or static retention-defective, A-ALP mutant indicate a role for Vps35p in retrieval. Wild-type (SNY17) and vps35Δ (SNY79) strains carrying plasmids expressing A-ALP (pSN55; lanes 1–6), A(Δ2–11)-ALP (pAH49; lanes 7–12), or A(F85A; F87A)-ALP (pSN100; lanes 13–18) were propagated at 30°C, radioactively pulsed for 10 min and chased, and at the indicated times wild-type or mutant A-ALP was immunoprecipitated. Samples were analyzed using SDS-PAGE and fluorography.