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A-ALP is mislocalized to the vacuole in a vps35–101 strain but exhibits Golgi staining in a vps35–108 strain. vps35–101 (SNY79/pLS12/pAH16) and vps35–108 (SNY79/pPB7–010.4/pAH16) strains were fixed, spheroplasted, and costained with antibodies against A-ALP and Vma2p. After treatment with fluorochrome-conjugated secondary antibodies, the cells were viewed by DIC optics and by epifluorescence through filters specific for FITC and Texas Red.
