Table 1.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pAH16 | pRS314 containing a DNA fragment encoding A-ALP | This study |
| pSN54 | pRS313 containing a DNA fragment encoding A-ALP | Nothwehr et al. (1993) |
| pSN55 | pRS316 containing a DNA fragment encoding A-ALP | Nothwehr et al. (1993) |
| pAH49 | pSN55 with codons 2-11 of the A-ALP construct deleted | This study |
| pSN100 | pSN55 with the Phe85 and Phe87 codons of A-ALP mutated to Ala | Nothwehr et al. (1993) |
| pLS13 | VPS35 gene in pRS316 | This study |
| pLS12 | vps35-101 allele in pRS316 | This study |
| pPB7-9.7 | vps35-103 allele in pRS316 | This study |
| pPB7-10.1 | vps35-104 allele in pRS316 | This study |
| pPB7-42.8 | vps35-105 allele in pRS316 | This study |
| pPB7-108.5 | vps35-107 allele in pRS316 | This study |
| pPB7-010.4 | vps35-108 allele in pRS316 | This study |
| pHY5 | pRS316 containing the VPS27 gene under GAL1 promoter control | T. H. Stevens |
| pPB6 | vps35Δ∷URA3 allele in Bluescript KS+ | This study |
| pGPY55 | vps35Δ∷HIS3 allele in a CEN plasmid | Paravicini et al. (1992) |