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Incorporation of palmitate by αz mutants. CHO-K1 cells, transiently transfected with αz mutants, were incubated with 0.75 mCi/ml [3H]palmitic acid for 2 h. αz was immunoprecipitated from total cell extracts with the EE mAb followed by SDS-PAGE. Identical gels were treated with either 1 M Tris (pH 7.0) or 1 M hydroxylamine (pH 7.0) for 12 h. Gels were then processed for fluorography (19-d exposure).
