Figure 6.
Effect of coexpressing βγ on activation of HA-MAPK by αz-G2A and αz-G2AC3A. D2R was transiently transfected into CHO-K1 cells with vector, αz-G2A, αz-G2A plus β1 and γ2, αz-G2AC3A, or αz-G2AC3A plus β1 and γ2. Cells were serum starved for 24 h and pretreated with PTX for 4 h before stimulating with serum-free medium (basal) or 10 μM quinpirole for 7 min. HA-MAPK activity was assayed as described in MATERIALS AND METHODS. Bars, mean ± 2 SE of triplicate determinations. Similar results were obtained in two separate experiments.