Figure 3.

Detergent-induced modulation of kinases activities in right-side-out and inside-out GPI-enriched membrane vesicles. One milliliter of the pooled GPI-enriched membrane fractions defined in Table 1 was immunoselected with anti-Thy-1 (A) or anti-Lck (B) antibody-coated protein A/G beads. The bound vesicles were washed in detergent-free buffer and subjected to in vitro kinase assay with [γ-³²P]ATP as detailed in MATERIALS AND METHODS. Thy-1-associated kinase activities were revealed after 3 d of exposure to the film, whereas Lck-associated phosphorylations required 15 d of exposure.