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Detergent-induced enhancement of kinase activities in GPI-enriched membranes is further augmented by vanadate. Kinase activities in untreated GPI-enriched membranes from 25 × 106 cells were measured by in vitro phosphorylation as described in Figure 2C in the presence of indicated concentrations of vanadate without or with 0.1% TX-100 in the reaction mixture.
