Figure 5.
p53-inducible, impd transfectants are resistant to p53-dependent growth suppression. The indicated control p53-inducible, vector transfectant (tC-2, tC-4) and impd transfectant (tI-1, tI-3, tI-5) cell lines were grown for 2 d under standard conditions in normal culture medium (0 Zn) or medium containing 75 μM ZnCl2 (75 Zn) to induce wild-type p53 expression. The graph shows representative growth data plotted as the mean of triplicate cell counts. Error bars denote the SD of the data. Growth data were fit to the ideal exponential growth equation to determine the doubling times shown in the inset table. 75/0, the ratio of the doubling time of p53-induced (75 Zn) cells to that of noninduced cells (0 Zn).