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. 1998 Jan;9(1):29–46. doi: 10.1091/mbc.9.1.29

Figure 5.

Figure 5

Cells harboring mob1 mutants arrest in late nuclear division. (A) Flow cytometric analysis of DNA content of a wild-type yeast strain grown at 25°C or 37°C for 3 h. The biphasic peaks represent cells with G1 (1N) and G2/M (2N) DNA content. (B) Flow cytometric analysis of DNA content of yeast harboring the mob1–77 allele grown at 25°C or 37°C for 3 h. At 37°C cells harboring mob1–77 mutation arrest as large budded cells with G2/M DNA content. Percent large-budded (LB) cells are noted. (C) Arrested mob1–77 cells (3 h at 37°C) were stained with anti-tubulin antibody (left panels) and 4,6-diamidino-2-phenylindole (right panels). The cells arrest with long mitotic spindles and separated chromatin masses. Three representative cells are shown.