Figure 2.

Effects of diamino compounds on protein synthesis and HA transport in CHO and NRK cells. To measure the extent of secretion by the trypsin sensitivity of HA, cells were infected with influenza virus and exposed to the indicated concentrations of drugs for 30 min at 37°C. The cells were incubated with Tran³⁵S label for 5 min and then incubated for 1 h in the absence of radioactive medium. The sensitivity of HA to trypsin was assessed as described in MATERIALS AND METHODS. To measure protein synthesis, uninfected cells were exposed to drugs and incubated for 1.5 h at 37°C. Tran³⁵S label was added during the last 30 min. The cells were lysed, and acid-insoluble radioactivity was measured.