Figure 3.

Effect of CBM on the binding of β-COP to Golgi-enriched membranes. Golgi-enriched membranes and cytosol were incubated together (Complete) under conditions to promote coatomer binding as described in MATERIALS AND METHODS. Omissions (−) and additions (+) to the reaction mixtures are indicated. Membrane-associated β-COP was detected by immunoblotting. The numbers below the bands represent a quantitative analysis of the band intensities as a percent of the complete reaction mixture (lane 3).