Figure 7.

The effects of CBM on the transport of HA from the ER to the Golgi and from the Golgi to the plasma membrane in CHO cells. Influenza virus–infected CHO cells were either untreated (B and C, ○) or treated with CBM (B and C, ●), radiolabeled, and then chased for the indicated times. After trypsin treatment, the cells were solubilized, and HA molecules were immunoprecipitated, treated with endoglycosidase H, and resolved in 10.5% SDS-polyacrylamide gels (A). Transport to the plasma membrane (B) and to the Golgi (C) was determined by trypsin sensitivity and endoglycosidase H resistance, respectively, as described in MATERIALS AND METHODS.