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. 1999 Apr;10(4):921–933. doi: 10.1091/mbc.10.4.921

Figure 8.

Figure 8

Reformation of the Golgi complex in cells first treated with brefeldin A and CBM followed by removal of the brefeldin A but retention of the CBM. NRK cells grown on glass coverslips were incubated with 2 mM CBM and 5 μg/ml brefeldin A for 30 min. Brefeldin A was then removed, but 2 mM CBM was retained. After a further 30 min incubation, the cells were fixed and prepared for immunofluorescence microscopy with anti-mannosidase II antibody.