Figure 4.

Absence of erythrocyte β-spectrin in E16 muscle fibers. Cryosections of E16 rat hindlimb were double-labeled with polyclonal antibodies to the extreme C-terminal sequence of erythroid β-spectrin (βIΣ1, panel A) and monoclonal antibodies to desmin (panel B), to identify the muscle cells, followed by rhodamine-conjugated anti-rabbit and FITC-conjugated anti-mouse secondary antibodies. The result shows that the desmin-positive muscle cells seen in E16 hindlimb do not contain the erythrocyte isoform of β-spectrin. Bar, 20 μm.