Figure 5.

Presence of β-spectrin, α-fodrin, and β-fodrin at the sarcolemma of embryonic rat skeletal muscle. Cross-sections of E16 (A–C, A′-C′) and E19 (D–F, D′–F′) rat diaphragm were labeled with isoform-specific polyclonal antibodies to β-spectrin (A and D), α-fodrin (B and E), and β-fodrin (C and F) along with monoclonal antibodies to desmin (A′–F′). The results show that all three subunits are expressed in embryonic rat skeletal muscle cells, where they assemble at or near the sarcolemma. Bar, 20 μm.