Table 3.
Effects of taxol on parameters of microtubule dynamic instability in living human tumor cells
| Parameter | Cell type and taxol concentration
|
|||
|---|---|---|---|---|
| A-498 kidney control | A-498 kidney + 100 nM | Caov-3 ovarian control | Caov-3 ovarian + 30 nM | |
| Number of microtubules | 40 | 26 | 36 | 34 |
| S-length (μm)a | 0.97 ± 0.7 | 0.71 ± 0.2b | 1.41 ± 1.0 | 1.18 ± 1.2 |
| S-duration (s) | 7.43 ± 4.7 | 7.53 ± 4.5 | 8.25 ± 4.8 | 9.62 ± 4.7 |
| G-length (μm)a | 0.87 ± 0.7 | 0.57 ± 0.1c | 1.23 ± 1.1 | 0.97 ± 0.65 |
| G-duration (s) | 7.01 ± 4.0 | 6.35 ± 3.5 | 9.84 ± 8.1 | 9.88 ± 4.7 |
| Pause duration (s) | 12.78 ± 13.6 | 22.29 ± 18.3d | 14.5 ± 15.6 | 16.9 ± 16.5 |
| Pause (% of total time) | 60.7 | 79.6 | 62.9 | 66.7 |
| Growth (% of total time) | 18.9 | 10.8 | 23.9 | 19.5 |
| Shortening (% of total time) | 20.4 | 9.6 | 13.2 | 13.8 |
| Catastrophe frequency (events/s) | 0.033 | 0.014 | 0.018 | 0.017 |
| Rescue frequency (events/s) | 0.120 | 0.137 | 0.103 | 0.093 |
| Catastrophe frequency (events/μm) | 1.13 | 1.26 | 0.506 | 0.765 |
| Rescue frequency (events/μm) | 0.923 | 1.46 | 0.604 | 0.755 |
| Dynamicity (μm/min) | 3.01 | 1.12 | 3.13 | 2.17 |
The values are expressed as mean or mean ± SD.
a
S, Shortening; G, growing.
b
Values significant at 95% confidence level (Student’s t test).
c
Values significant at 99% confidence level (Student’s t test).
d
Values significant at 99.9% confidence level (Student’s t test).