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. 1998 Jan;9(1):63–73. doi: 10.1091/mbc.9.1.63

Figure 1.

Figure 1

GST-Cdc18 is functional in vivo and induces rereplication when overexpressed. (A) Cells expressing GST (strain AL1853) or GST-Cdc18 (strain AL1854) from the thiamine-repressible nmt1 promoter were photographed after 2 d of incubation at 30°C on agar medium lacking thiamine. Cells expressing GST grew well, whereas cells expressing GST-Cdc18 underwent several divisions and then became cdc arrested. (B) DNA content was measured by FACS in cells grown in liquid medium lacking thiamine. Note that the nmt1 promoter becomes active approximately 10 h following depletion of thiamine from the growth medium, reaching maximum activity at approximately 16 h (Maundrell, 1993). 2C and 4C positions were determined using wild-type haploid and diploid strains growing exponentially in YES medium at 30°C.