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GST-Cdc18 interacts with Orp2, Cdc2, and B-type cyclins. GST and GST-Cdc18 expression was induced for 18 h. Lysates from those cells, GST (lane 1) and GST-Cdc18 (lane 2), were incubated with GSH-Sepharose beads. The bound proteins were separated by SDS-PAGE and analyzed by immunoblotting with antibodies specific for GST, Orp2, Cdc2, Cdc13, or Cig2, as indicated. GST-Cdc18 copurified specifically with Orp2, Cdc2, Cdc13, and Cig2.
