Figure 7.
Effect of α-, β-, methyl-β-, and γ-cyclodextrins on transferrin endocytosis. HEp-2 cells were washed briefly in HEPES-buffered medium and incubated with and without α-, β-, methyl-β-, or γ-cyclodextrins (10 mM) for 15 min at 37°C. 125I-labeled transferrin was then added to the cells, and the amounts of endocytosed transferrin were measured as described in MATERIALS AND METHODS. The error bars show deviations between duplicates.