Figure 8.

Reversal of MβCD-induced inhibition of transferrin endocytosis in the absence (A) or presence (B) of lovastatin. In both cases MDCK II cells were washed briefly in HEPES-buffered medium and incubated with and without MβCD (10 mM) for 15 min at 37°C. The cells were then incubated in HEPES-buffered medium with and without 5% FCS or water-soluble cholesterol (400 μg/ml) in the presence or absence of lovastatin (5 μg/ml) from 15 min to 3 h (A) or for 3 h (B) before ¹²⁵I-labeled transferrin was added. The amounts of endocytosed transferrin were measured as described in MATERIALS AND METHODS. The error bars show deviations between duplicates.