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. 1998 Jan;9(1):75–88. doi: 10.1091/mbc.9.1.75

Figure 1.

Figure 1

Schematic diagram of the myoB expression plasmids. The pBluescript-based plasmid pDTb2 contains the 3.6-kb full-length myoB gene along with 1.22 kb of 5′ noncoding region that serves as promoter (BPr.) and 0.45 kb of 3′ noncoding sequences (T) that are likely to encompass the terminator region of the myoB gene. The pGEM7-based plasmid pDTb18 contains the 0.3-kb actin 15 promoter (hatched box) upstream of the full-length 3.6-kb myoB coding sequence and 0.45 kb of 3′ noncoding sequence (T). The plasmid pDTb42 contains the 0.3-kb actin 15 promoter (hatched box) upstream of a mutant 3.7-kb myoB gene containing a serine to alanine change at amino acid 332 (nts. 1361–1363). The vector pDTb39 is a pLittle-based vector that contains the 0.3-kb actin 15 promoter (hatched box) upstream of a truncated form of the myoB gene (myoB/SH3−) that lacks the 3′ 0.16 kb of coding sequence encompassing the SH3 domain and 0.45-kb myoB terminator. The myoB/SH3− gene is immediately adjacent to the 2.2-kb Neo resistance cassette (gray box) whose transcription is driven by the actin 6 promoter. The arrows represent direction of myoB transcription. The vector backbone is not illustrated in these diagrams.