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Expressed myoB, myoB/SH3−, and myoB-S332A all exhibit ATP-sensitive binding to actin. The Mg-ATP release of the myoB heavy chain from the Triton-insoluble fraction obtained from a rigor cytoskeleton was analyzed using immunoblotting. Shown are equal amounts of the supernatant (S) and pellet (P) fractions derived from the Ax3 myoB, S332A-myoB (S332A), and myoB/SH3− (SH3−)-expressing cells.
