Figure 2.

Expression of myoB in myoB⁻ cells rescues the streaming defect. (A) Western blot analysis shows that myoB is expressed in the B⁻ rescue cell line. The position of the M_r 116,000 marker is indicated on the left of the Western blot. The levels of expression of the 125-kDa myoB heavy chain in equal numbers of Ax3 control (Ax3), myoB⁻ mutant, and the myoB-expressing cell line (B- rescue) are shown. The same cell lines were analyzed in the streaming assay and photographed at 8 h after starvation (B–D). Bar, 50 μm.