Figure 3.

Freeze fracture immunoelectron microscopy. (A–C) src^ts NRK cell cultured at the permissive temperature. (A and B) Labeling by PY14 occurred not only in caveolae of normal shape but also in IMP-free flat areas of various sizes and shapes (arrowheads). (C) Similar labeling was observed with sc-894. (D) In normal NRK cells, virtually all the labeling by sc-894 was seen in deep (single large arrows) and shallow (double small arrows) caveolae, and IMP-free flat areas were scarce. Bar, 100 nm (A, C, and D), 85 nm (B).