Figure 7.

Biochemical characterization of tyrosine-phosphorylated caveolin-1. (A) Solubility in Triton X-100. The reaction of PY14 in SR-3Y1 as well as that of sc-894 in 3Y1 occurred intensely for the Triton X-100–insoluble material (I) but only weakly for the Triton X-100–soluble one (S). (B) Oligomer formation. Fractions of sucrose density gradient ultracentrifugation were subjected to SDS-PAGE, and caveolin-1 was detected by Western blotting using sc-894 and PY14. Both 3Y1 and SR-3Y1 showed a positive reaction between 150 and 400 kDa. Locations of molecular mass marker proteins are indicated by arrows. (C) Association with caveolin-2. Immunoprecipitates obtained with sc-894 from both 3Y1 and SR-3Y1 cells are positive for caveolin-2.