Figure 8.

Western blots of mutant caveolin-1 whose tyrosine 14 was replaced with asparagine. (A) The lysates of transfected 3Y1 and SR-3Y1 cells were reacted with anti–c-myc tag antibody. The band of 3Y1 indicates the position of nonphosphorylated mutant caveolin-1α. At least one additional band is seen above the α isoform in the SR-3Y1 sample. (B) The lysate of transfected SR-3Y1 cells was precipitated with sc-894 and dephosphorylated by alkaline phosphatase before electrophoresis and Western blotting by anti–c-myc antibody. By dephosphorylation, the reaction with the band above the α isoform was abolished.