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Normal NRK cells treated with vanadate. (A) Western blotting shows that the reaction with PY14 occurs as a single band at 22 kDa (at 2 h) and as three bands at 22, 23–24, and 25 kDa (at 8 h). (B and C) Immunofluorescence micrographs of normal NRK cells before (B) and after (C) the vanadate treatment. Caveolin-1 was localized as peripheral patches in untreated cells, but they disappeared after the treatment. Bar, 10 μm.
