Figure 2. Phenotypic and functional characteristics of expanded CliniMACS isolated Treg.

Cell populations were expanded with polyclonal stimulus or alloantigen stimulus in the presence of exogenous IL-2. Data from a typical experiment are shown (N = 3). (A) Cell surface expression of CD25 and intracellular expression of FoxP3. (B) Proliferative capacity upon restimulation with allogeneic PBMC (same donor as in alloantigen expansion) in the absence or presence of exogenous IL-2, measured at day 3 of culture. (C) Suppressive capacity in co-cultures of autologous naïve CD4^posCD25^neg Tresp stimulated with allogeneic PBMC (same donor as in alloantigen expansion). Significant differences are indicated by asterisks. In figure C, open and filled asterisks refer to Tconv (expanded with same stimulus as Treg populations) versus MiniMACS or CliniMACS^8/25, respectively.