Figure 3. Cryopreservation of CliniMACS Treg affects suppressive capacity, which can be restored by activation.

Suppressive capacity of cryopreserved CliniMACS Treg in co-cultures of autologous naïve CD4^posCD25^neg Tresp stimulated with allogeneic PBMC. Data from a typical experiment are shown (N = 2–4). Prior to co-culture suppression assay, indicated cell populations were expanded for 10 days in the presence of exogenous IL-2 and allogeneic PBMC (same donor as in co-culture suppression assay). Significant differences are indicated by asterisks. Open and filled asterisks refer to cryopreservation versus cryopreservation plus 4 days rest or cryopreservation plus expansion, respectively.