Figure 1. Induction of IFN-λ2 genes in virus-infected lung and liver of IFNAR1^0/0 mice.

Animals were either infected by the intranasal route with 10⁶ pfu of influenza A virus strain SC35M-ΔNS1 or PR8-ΔNS1, or else by the intraperitoneal route with 10 pfu of hepatotropic THOV-ΔML. Animals treated with plain buffer served as negative controls. At 17 hours post infection, the influenza virus-infected mice were killed and the lungs were removed for analysis. The liver of the THOV-infected mouse was harvested when the animal was severely diseased at 72 hours post infection. RNA samples from the organs were reverse transcribed and analyzed by PCR for transcripts of the indicated genes.