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. Author manuscript; available in PMC: 2008 Nov 21.
Published in final edited form as: J Am Chem Soc. 2007 Oct 31;129(46):14336–14347. doi: 10.1021/ja074650f

Table 2.

Kinetic Data for Esterase MceDa

Ent MGE DGE lin-Ent lin-MGE
kcat(min−1) 207 ± 10 564 ± 20 910 ± 33 139 ±4 30 ± 2.5
Km(μM) 2.5 ± 0.4 45 ±9 29 ± 3 1.7 ± 0.2 45 ±9
kcat/Km(min−1μM−1) 83 34 31 82 0.67
[Fe(Ent)]3− [Fe(MGE)]3− [Fe(DGE)]3− C10-C6′-MGE C10-C4′-MGE
kcat(min−1) 43 ± 1.3 39 ± 2 29 ± 1.5 606 ± 53 767 ± 93
Km(μM) 2.5 ± 0.3 7.7 ± 1.4 7.2 ± 1.1 15.5 ± 3.6 26 ± 7
kcat/Km(min−1μM−1) 17 5 5 39 29.5
a

All reactions were carried out at room temperature (75 mM Hepes, pH 7.5).