Fig. 8.

Dose–dependent regulation of Cre-mediated recombination by decreasing tamoxifen. Immunohistochemistry for GFP was performed. (a–c) GFAP-CreER ^TM A; Z/EG double transgenice mouse injected with a single 1 mg/40 g dose of tamoxifen. (d–f) GFAP-CreER ^TM A; Z/EG double transgenice mouse injected with a single 3 mg/40 g dose of tamoxifen. (g–i) GFAP-CreER ^TM A; Z/EG double transgenice mouse injected with a single 9 mg/40 g dose of tamoxifen. Brain regions presented are sagittal sections through the cerebellum (panels a, d, g), cerebral cortex (panels b, e, h) and hippocampus (panels c, f, i). Scale bar is 0.5 mm and applies to all panels