Fig. 1.

Phenotype and IL-12p70 production after DC maturation with different cocktails. Immature DC were matured for 48 h with different maturation cocktails, maturation markers were evaluated by flow cytometry and IL-12p70 secreted was measured in the supernatant by ELISA. a DC from three different donors matured with the conventional cytokine cocktail (TNFα, IL-6, IL-1α and PGE₂), TLR3 ligand poly(I:C), TLR7/8 ligand (R848) or both. Upper panels show the different maturation cocktails used. Horizontally the expression of CD80, CD83, CD86, CCR7 and MHC class II from one representative donor measured by flow cytometry is shown (bold line). The thin line represents the isotype control. The lower panel shows the IL-12p70 production in the supernatant measured by ELISA. b DC from different donors were matured with the conventional cytokine cocktail (TNFα, IL-6, IL-1β and PGE₂), or poly(I:C) or R848 or poly(I:C)/R848 in the combination with TNFα, IFNα, IFNγ with and without PGE₂. IL-12p70 production was measured after 48 h. Per condition each symbol represents one donor. Means are shown for each maturation cocktail and were compared to cDC (*  p < 0.05), comparison of the different cocktails with TLR-DC were significant with all the different cocktails. There is no significant difference between PGE₂-TLR-DC and the TNFα/IFNα/IFNγ-PGE₂-TLR-DC