Fig. 3.

CCR7 expression and CCR7-mediated chemotaxis of cDC, TLR-DC and PGE₂-TLR-DC. DC from three different donors were matured with the conventional cytokine-cocktail or with poly(I:C), R848 with or without PGE₂. a CCR7 expression of the different matured DC and CD80, CD83, CD86 and MHC class II expression of PGE₂-TLR-DC measured by flowcytometry is shown. b Chemotaxis was determined by the number of cells that had migrated into the lower compartment of a transwell system containing increasing concentrations of CCL21, counted in 1 minute by flow cytometry. Kinesis was determined by the number of cells that had migrated into the lower compartment when CCL21 is present in both compartments in the same concentration. The graph shown here is representative for three different donors. The error bars show the standard deviation