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. 2008 Mar 6;57(11):1589–1597. doi: 10.1007/s00262-008-0489-2

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© The Author(s) 2008

Open AccessThis is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Fig. 4 — IL-12p70 production upon secondary stimulation. DC were matured with the conventional cytokine cocktail (TNFα, IL-6, IL-1β and PGE₂), or TLR ligands (poly(I:C)/R848) or TLR ligands in combination with PGE₂ for 48 h and then cultured for another 24 h with or without PBL or CD40L-trimers. IL-12p70 production was measured in the supernatant by ELISA. Per condition, each symbol represents the mean for each donor. Means are shown for each maturation cocktail (open symbols cDC, gray symbols TLR-DC, black symbols PGE₂-TLR-DC, NS non significant)