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. Author manuscript; available in PMC: 2008 Aug 26.
Published in final edited form as: Biochemistry. 2006 Nov 14;45(45):13537–13542. doi: 10.1021/bi060938q

Figure 5.

Figure 5

DNA strand exchange catalyzed by the RecA and RecA-FD proteins. Strand exchange reactions were performed as described (see EXPERIMENTAL PROCEDURES) and include 20 μM φX174 ss- and dsDNAs (concentration in bases), 6.0 μM RecA or RecA-FD proteins, 2.0 μM SSB and 3.0 mM ATP. Samples were removed at the indicated times and substrates and products were separated by agarose gel electrophoresis. Products of the reaction include joint molecule intermediates (JM) and final product nicked circles (NC).