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. 2004 Jul 21;24(29):6621–6628. doi: 10.1523/JNEUROSCI.1474-04.2004

Figure 4.

Figure 4.

Bath application of the NO analog SNAP results in a reversible, KT5823-sensitive potentiation of On bipolar cell responses. A, On bipolar cells with stabilized responses were bathed in SNAP (closed symbols) for 6 min (time on and off are indicated by arrows) and stimulated with 150 msec sim-flashes every 30 sec (n = 7). Data points were binned into 1 min intervals, and averages were plotted over 16 min. Responses started to potentiate 1 min after SNAP application and lasted for ∼3 min after removal (p < 0.001 at 4 min, compared with baseline and KT5823). The amplification was reversible, returning to amplitudes between 10 and 20% above baseline. The addition of KT5823 to the recording pipette (n = 7) completely blocked the SNAP-induced potentiation (open symbols). B, Example of single-cell traces illustrating the potentiating effect of SNAP compared with a control trace recorded before SNAP was applied. C, Data summarized in histogram form. The bar labeled “Control” represents the normalized response after SNAP has been washed out.