| SEREX |
Many clones, clone for gene automatically available |
Denatured epitopes, laborious, large amounts of serum, requires separate validation step |
Antigen discovery |
| Western Blotting |
Reproducible |
Large amounts of serum, requires separate steps to identify and validate antigens |
Antigen Discovery |
| Phage display |
Large number of clones, little serum required, ability to refine selection with multiple rounds, clone for gene automatically available |
Partial gene products, gene overrepresentation, requires separate validation step |
Antigen discovery |
| Cell Fractionation |
Retains post translational modification, multiple arrays allow for screening for fractions that are informative for cancer, do not need to have cloned gene or library for screening |
Tedious, must identify the relevant antigen in potentially complex fraction, may need to clone gene for identified protein, requires separate validation step, post translational modification may not be preserved in the validation step |
Antigen discovery |
| ELISA |
Reproducible, useful for comparing many serum samples |
Tedious to produce protein for the assay. |
Validation |
| Candidate antigen protein microarrays |
Screen many candidate antigens, validation and antigen discovery can be done simultaneously, very little serum required, clone for gene automatically available |
Arrays can be challenging to produce, novel techonology |
Antigen discovery and validation |
