Figure 3.

Comparison of the effect of rapamycin on eIF4G protein levels versus r-protein mRNA levels. (A) Top, protein samples were prepared, and Western blot analysis was performed using polyclonal antisera raised against eIF4G or eIF4E. Bottom, alternatively, total RNA was prepared and probed for the indicated mRNAs by Northern blot analysis. (B) Quantitation of data from A compares the decline in eIF4G protein levels (normalized to eIF4E protein levels) with the decline of RPL32 mRNA levels (normalized to ACT1 mRNA) after rapamycin treatment.