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. 1998 Jan;9(1):117–130. doi: 10.1091/mbc.9.1.117

Figure 2.

Figure 2

SRP-independent translocation in the reticulocyte lysate system. Ribosome-pPL86 complexes were assembled in a reticulocyte lysate system. The RNCs were resuspended in the absence of the protease inhibitor cocktail. Peptidyl-tRNA was precipitated from an aliquot of the NAC-depleted and mock-depleted ribosome-pPL86 complexes with CTABr. Additional aliquots of the NAC-depleted and mock-depleted ribosome-pPL86 complexes were incubated in the presence or absence of 3 eq of PK-RM. The assays were chilled on ice before incubation with proteinase K. (A) Proteinase K resistant pPL86 was resolved from the ribosome-protected fragment (30 mer) by PAGE in SDS. (B) The percent translocation was calculated as (100 × protease resistant pPL86)/(CTABr precipitated pPL86).