Figure 1.

Schematic diagram of Myo2p, Myo2p tail construct, and anti-Myo2p antibodies. (A) The domain structure of the full-length Myo2p protein. Lines above the schematic indicate regions of Myo2p used to produce the motor- and tail-domain antibodies used in this study. The DNA encoding the tail domain of Myo2p (B), which did not include any of the region predicted to form α-helical coiled coil, was overexpressed behind the GAL1 promoter to produce the yeast strain MYO2DN. Conventionally, the entire region after the neck domain is referred to as the “tail”; however, in this work we refer to the region after the coiled coil as the “tail”.