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Sec4p localization in MYO2DN. Wild-type (WT) and MYO2DN cells were grown in glycerol (A and B) and shifted to galactose for 45 min (C and D) or 3 h (E and F), fixed, and stained using anti-Sec4p antibody. Sec4p staining was polarized in wild-type cells or MYO2DN cells grown in glycerol (n = 200) or galactose for 45 min (n =400). After 3 h in galactose only 2% (n =409) of MYO2DN cells observed had polarized Sec4p staining. Scale bar, 5 μm.
