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Overexpression of the tail of Myo2p causes the endogenous Myo2p to delocalize. Wild-type (WT) and MYO2DN cells were grown in glycerol (A and B) and shifted to galactose for 45 min (C and D), fixed, and stained with anti-Myo2p head antibody. Myo2p was localized to the bud in wild-type and MYO2DN cells grown in glycerol (n = 200) and in wild-type cells grown in galactose for 45 min (n = 420), but was delocalized in MYO2DN cells grown in galactose for 45 min (n = 402). Scale bar, 5 μm.
